APPLICATION OF TRICHINELLA PAPUAE CRUDE ANTIGEN IN IMMUNODIAGNOSIS OF HUMAN TRICHINELLOSIS PAPUAE AND IDENTIFICATION OF IMMUNODOMINANT ANTIGENS
Keywords:
Trichinella papuae, ELISA, immunoblot, liquid chromatographymass spectrometry, serodiagnosisAbstract
Immunodiagnosis of human trichinellosis papuae by indirect ELISA and immunoblot was developed using Trichinella papuae muscle L1 larva crude worm antigens (TpaL1-CWA) and tested with human sera of trichinellosis papuae (n = 18), healthy control (n = 26) and other parasitic diseases (n = 160). IgG2- and IgG3-indirect ELISAs failed to elicit positive results, total IgG-, IgG1- and IgG4-ELISAs demonstrated sensitivity of 100, 100 and 66.7%, respectively and specificity of 90.3, 89.8 and 66.7%, respectively. Western blotting of TpaL1-CWA revealed predominant immunoreactive antigens of 31, 45 and 66.2 kDa against total IgG and IgG1 of trichinellosis papuae sera, which, except for 31 kDa antigen, showed cross-reactivity with sera of several other parasitic diseases. Specificity, sensitivity, positive predictive value, and negative predictive value of T. papuae 31 kDa in IgG1-immunoblot were 100%. Mass spectrometry analysis of the 31 kDa antigen revealed the presence (in decreasing order of identity score) of 3-hydroxyacyl-CoA dehydrogenase, GLIPR1-like protein 1, tissue-type plasminogen activator, hypothetical protein T10_8058, and hypothetical protein T10_12289. Future studies should focus on evaluating the most appropriate candidate 31 kDa antigen to prepare recombinant protein for application in trichinellosis papuae immunodiagnosis.
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- 2022-06-01 (2)
- 2022-05-30 (1)