DETERMINATION OF PARAQUAT IN PLASMA AND URINE BY MICELLAR LIQUID CHROMATOGRAPHY

Authors

  • Ms. Warin Leelakunakorn Mahidol University
  • Dr. Jatupon Krongvorakul Mahidol University
  • Dr. Saranya Auparakkitanon Mahidol University

Keywords:

medical decision level, micellar liquid chromatography, paraquat, plasma, urine

Abstract

The study aimed to apply micellar liquid chromatography (MLC) to detect paraquat levels in human plasma and urine samples at a medical decision level for suspected poisoning, which is not achieved by the current ion-pairing LC method. Paraquat in (acid-treated) human plasma and urine samples was analyzed using C18 Eclipse XDB column maintained at 30°C and an aqueous micellar mobile phase (pH 3.0) composed of 50 mM sodium dodecyl sulfate, 15% (v/v) acetonitrile, 0.5% (v/v) diethylamine, and 9% (v/v) n-butanol, with paraquat monitored by measuring absorbance at 258 nm. The limit of detection of plasma paraquat (PPQ) and urine paraquat (UPQ) was 0.02 and 0.05 µg/ml respectively; and the limit of quantification was 0.05 and 0.16 µg/ml respectively. Dynamic range for both PPQ and UPQ was achieved at 0.16-100 µg/ml and recovery of spiked (2-10 µg/ml) PPQ and UPQ samples ranged from 100.4-102.9%. Within run repeatability of PPQ and UPQ, detection was obtained at 1.8 and 3.5% relative standard deviation (RSD) respectively and within laboratory repeatability of 4.16 and 7.31% RSD respectively, with a turn-around time of eight minutes. In conclusion, the MLC method was capable of detecting paraquat at a medical decision level for suspected poisoning, namely, 0.2 and 0.9 µg/ml in plasma and urine respectively.

Author Biographies

  • Ms. Warin Leelakunakorn, Mahidol University

    Research Associate

    Department of Pathology, Faculty of Medicine Ramathibodi Hospital, Mahidol University, Bangkok, Thailand

  • Dr. Jatupon Krongvorakul , Mahidol University

    Lecturer

    Department of Pathology, Faculty of Medicine Ramathibodi Hospital, Mahidol University, Bangkok, Thailand

  • Dr. Saranya Auparakkitanon, Mahidol University

    Associate Professor

    Department of Pathology, Faculty of Medicine Ramathibodi Hospital, Mahidol University, Bangkok, Thailand

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Published

2023-05-29

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