RESEARCH NOTE FIELD EVALUATION OF A DIPSTICK DYE IMMUNOASSAY IN LARGE-SCALE SCREENING OF HUMAN SCHISTOSOMA JAPONICUM INFECTIONS IN CHINA

Efficiency of DDIA for detecting S. japonicum infection

Authors

  • Rujin Xu
  • Yueqin Chen First People's Hospital of Linping District
  • Fang He
  • Yenan Shen
  • Chunyan Qian

Keywords:

Schistosoma japonicum, diagnostic efficacy, dipstick dye immunoassay, schistosomiasis japonica screening, China

Abstract

Schistosomiasis japonica remains a major public health concern in China. Diagnosis is central to control and elimination of schistosomiasis in China. As parasitological techniques are laboratory-intensive, time-consuming and insensitive in low-endemicity regions, compounded with poor compliance, immunodiagnostics with high sensitivity and specificity provide an alternative tool. Here we examined the efficacy of a dipstick dye immunoassay (DDIA) in a large-scale screening of human Schistosoma japonicum infection among adult residents (n = 404) of a village located a schistosomiasis endemic region near Poyang Lake, Jiujiang, Jiangxi Province in comparison with an enzyme-linked immunosorbent assay (ELISA), an indirect hemagglutination assay (IHA) and a miracidium hatching test, the gold standard. A 13.4% prevalence of S. japonicum infection was detected using the gold standard test, with a diagnostic sensitivity, specificity, positive predictive value and negative predictive value of 94.4, 67.7, 31.1, and 98.8%, respectively for DDIA; 87.0, 65.1, 27.8, and 97.0%, respectively for ELISA and 75.9, 72.9, 30.1, and 95.1%, respectively for IHA. Assays of serum samples of healthy subjects (n = 418) who resided in non-endemic regions and had never traveled in schistosomiasis-endemic areas revealed a diagnostic specificity of DDIA, ELISA and IHA of 98.1, 97.4 and 98.8%, respectively. In conclusion, DDIA proved to be a simple and rapid immunodiagnostic method with high sensitivity and specificity as evaluated in a large-scale screening of human S. japonicum infection in an endemic region of China.

Downloads

Published

2022-08-20

Similar Articles

1-10 of 82

You may also start an advanced similarity search for this article.