SMALL RNA INTERFERENCE INHIBITION OF UBIQUITIN-SPECIFIC PROTEASE 14 GENE EXPRESSION IN INTRAHEPATIC CHOLANGIOCARCINOMA PRIMARY CELLS

Ubol Chuensumran1, Tasanee Panichakul2, Thun Ingkakul3 and Poom Adisakwattana4

Abstract

Studies on genomic instabilities of intrahepatic cholangiocarcinoma (ICC) from Thai patients have shown 52% variations in a specific region of ubiquitin-specific protease 14 gene (USP14) located on 18p11.32. Inhibition of USP14 expression in three different primary cells cultured from resected ICC tissues by transfecting with double-strand RNA interference targeting exon 16 region of USP14 mRNA resulted in 6-50% reduction in mRNA levels, with cells with low USP14 expression being more affected. These findings lend support to a potential in developing strategies against USP14 in ICC.