SMALL RNA INTERFERENCE INHIBITION OF UBIQUITIN-SPECIFIC PROTEASE 14 GENE EXPRESSION IN INTRAHEPATIC CHOLANGIOCARCINOMA PRIMARY CELLS

Ubol Chuensumran1, Tasanee Panichakul2, Thun Ingkakul3 and Poom Adisakwattana4

Authors

  • Ubol Chuensumran

Keywords:

intrahepatic cholangiocarcinoma, primary cell culture, RNAi, ubiquitin-specific protease 14

Abstract

Studies on genomic instabilities of intrahepatic cholangiocarcinoma (ICC) from Thai patients have shown 52% variations in a specific region of ubiquitin-specific protease 14 gene (USP14) located on 18p11.32. Inhibition of USP14 expression in three different primary cells cultured from resected ICC tissues by transfecting with double-strand RNA interference targeting exon 16 region of USP14 mRNA resulted in 6-50% reduction in mRNA levels, with cells with low USP14 expression being more affected. These findings lend support to a potential in developing strategies against USP14 in ICC.

Published

2020-10-26 — Updated on 2021-08-13

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